eclipse ti2 e n sim e fluorescence microscope Search Results


eclipse ti2 e n sim e fluorescence microscope  (Nikon)
99
Nikon eclipse ti2 e n sim e fluorescence microscope
Eclipse Ti2 E N Sim E Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ti2+e+n+sim+e+fluorescence+microscope/Objectives/pm37318230-258-17-23
Average 99 stars, based on 1 article reviews
eclipse ti2 e n sim e fluorescence microscope - by Bioz Stars, 2026-09
99/100 stars
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3d sim  (Nikon)
99
Nikon 3d sim
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
3d Sim, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ti2+e+n+sim+e+fluorescence+microscope/ECLIPSE+Ti2/pmc06414705-228-0-13
Average 99 stars, based on 1 article reviews
3d sim - by Bioz Stars, 2026-09
99/100 stars
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orca flash4.0 lt plus scmos camera  (Hamamatsu)
90
Hamamatsu orca flash4.0 lt plus scmos camera
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Orca Flash4.0 Lt Plus Scmos Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ti2+e+n+sim+e+fluorescence+microscope/orca+flash+4+0+camera/pm37318230-258-46-52
Average 90 stars, based on 1 article reviews
orca flash4.0 lt plus scmos camera - by Bioz Stars, 2026-09
90/100 stars
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lu n3 sim laser  (Nikon)
93
Nikon lu n3 sim laser
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Lu N3 Sim Laser, supplied by Nikon, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ti2+e+n+sim+e+fluorescence+microscope/LU-N4%2FN4S+4-laser+Unit%2C+LU-N3+3-laser+Unit/pm37256900-309-85-88
Average 93 stars, based on 1 article reviews
lu n3 sim laser - by Bioz Stars, 2026-09
93/100 stars
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Image Search Results


Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy (3D-SIM) allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="100%" height="100%">

Journal: mBio

Article Title: The Polar Organizing Protein PopZ Is Fundamental for Proper Cell Division and Segregation of Cellular Content in Magnetospirillum gryphiswaldense

doi: 10.1128/mBio.02716-18

Figure Lengend Snippet: Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy (3D-SIM) allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm.

Article Snippet: 3D-SIM (striped illumination at 3 angles and 5 phases) was performed on a Nikon Eclipse Ti2-E N-SIM E fluorescence microscope equipped with a CFI SR Apo TIRF AC 100×H NA1.49 oil lens objective.

Techniques: Time-lapse Microscopy, Expressing, Fluorescence, Microscopy, Epifluorescence Microscopy, Staining